Document Details

Document Type : Thesis 
Document Title :
CONSTRUCTION OF A COMPREHENSIVE TRANSPOSON LIBRARY OF THE PLANT GROWTH PROMOTING BACTERIUM ENTEROBACTER SP. SA187
بناء مكتبة ترانسبوزون شاملة لبكتريا Enterobacter sp. SA187 المحفزة لنمو النبات
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Plants surviving under stressed conditions are believed to benefit from associated microorganisms named plant growth promoting bacteria (PGPB). Enterobacter sp. SA187 is a model PGPB that was isolated from the root nodules of Indigofera argentea, Jazan region, Kingdom of Saudi Arabia. SA187 showed several PGPB traits for nutrient acquisition, hormone production, as well as enhancing the growth of crop plants under different abiotic stresses. A complete genome sequence of the SA187 showed different metabolic capacity with 4,347 predicted protein-coding DNA sequences (CDS). To identify the essential genes/ pathways involved in the beneficial SA187/ plant interaction and to correlate different phenotypes and functions to their genomic sequences and pathways, we constructed a saturated mutant library using Tn5 random mutagenesis. The generated library theoretically covered all the genome of SA187 with approximately 16,000 generated clones, all obtained mutants acquired the resistance to the antibiotic Kanamycin as a marker. The Tn5 insertion SA187 library was mapped using Sanger sequencing with specific primers designed for amplification of the flanking region of transposon sequence. Large number of genes were identified including genes involved in mobility, carbohydrates metabolism, surface appendages as well as protein with unknown function, but most importantly, the TrpCF mutant responsible for tryptophan biosynthesis, which eventually contributes to the synthesis of indole3-acetic acid (IAA). Overall, the generated mutagenesis library provides a toolbox to screen and identify the essential genes involved in the SA187 lifestyle as a free-living soil bacterium and as an endophytic bacterium within its host plant. 
Supervisor : Prof. Dr. Rashad Rizq Al-Hindi 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2020 AD 
Added Date : Wednesday, June 24, 2020 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عبد الرحمن بد الرشيد هاشمHashim, Abdulrahman AbdulrashidResearcherMaster 

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 46505.pdf pdf 

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