Document Details

Document Type : Thesis 
Document Title :
Effect of some Freshwater Herbs and Seagrasses Extracts on Selected Microbial Pathogens
تـأثير مستخلصات بعض أعشاب المياه العذبة والبحرية على سلالات ميكروبية ممرضة
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : The present study describes the antimicrobial, antioxidant, phytochemical analysis and cytotoxicity of the extracts of four freshwater herbs leaves were collected from Albahah and Taif cities and four seagrasses leaves were collected from different sites of Red Sea, Saudi Arabia. The collected samples were extracted with different solvents. The results showed that the tested freshwater herbs and seagrasses leaves extracts were active against selected microbial pathogens. The results indicated to Ethyl acetate leaves extract of J. rigidus and Ethanolic leaves extract of H. uninervis showed the highest activity against MRSA and P. aeruginosa, respectively, synergistic and antagonistic effects with different antibiotics, the lowest values of MIC and MBC and different concentrations in sugars and proteins of bacterial cells walls. While, Chloroform leaves extracts of V. anagallis-aquatica and H. uninervis showed the highest antifungal activity against A. niger, antagonistic effects with antibiotics, the lowest values of MIC and different concentrations in sugars, proteins and phosphates of fungal cella walls. Moreover, the freshwater herbs and seagrasses leaves extracts displayed antioxidant activity in DPPH assay with different percentages. Chemical analysis of tested freshwater herbs and seagrasses leaves extracts reported different active components that have biological activity. J. rigidus leaves extract appeared the highest percentages of cytotoxicity test against two types of cancer cells (HCT116 and MCF7) and one type of normal cell (BJ1). Finally, six random primers for bacteria were used in RAPD analysis of MRSA and P. aeruginosa and twelve random primers were used for A. niger fungi. The obtained results showed that tested extracts affected the two types of bacteria and fungi used in the study. RAPD analysis displayed two types of polymorphisms either absence of amplified fragments or induction of new fragments in the treated or untreated tested microorganisms. 
Supervisor : D. Nehad Mahmoud Gum Gumjee 
Thesis Type : Doctorate Thesis 
Publishing Year : 1440 AH
2019 AD 
Co-Supervisor : Prof. Abdulrahman Saeed Hajar 
Added Date : Monday, June 17, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
دعاء عبدالرشيد بخاريBukhari, Duaa AbdulrashidResearcherDoctorate 

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