Document Details

Document Type : Article In Journal 
Document Title :
DNA METHYLATION IN HUMAN GENES FOR SCHISTOSOMA-ASSOCIATED AND NON-SCHISTOSOMA-ASSOCIATED BLADDER CANCER
ميثلة الحمض النووي في الجينات البشرية لسرطان المثانة المرتبط بالبلهارسيا وغير المصاحبة لمرض البلهارسيا
 
Subject : DNA METHYLATION IN HUMAN GENES FOR SCHISTOSOMA-ASSOCIATED AND NON-SCHISTOSOMA-ASSOCIATED BLADDER CANCER 
Document Language : English 
Abstract : Aims: to analyze the usefulness of DNA methylation in eight candidate genes as tumor markers in bladder cancer of Schistosoma-associated and non-Schistosoma-associated bladder cancer. Methodology: MethyLight assay was utilized to investigate the DNA methylation status of eight cancer related genes using DNA extracted from paraffin-embedded (FFPE) tissues of Saudi patients with bladder cancer of both Schistosoma-associated and non-Schistosoma-associated. These genes include TIMP3, RASSF1A, SLIT2, SOCS1, RUNX3, NEUROG1, IGF2 and CACNA1G. Results: 85% of the investigated samples displayed detectable methylation level in one up to six genes. None of the 45 samples reacted positively to all genes. On other hand, only seven cases did not display any methylation. The correlations between methylation and the investigated genes were illustrated. Conclusion: SLIT2 was the most frequently methylated gene and none of the investigated cases showed methylation to all eight genes. Methylation in Saudi patients with Non-Schistosoma-associated bladder cancer was higher than the Schistosoma-associated bladder cancer. There is a need for further work covering panel of genes to correlate them with further factors related to the clinical and pathological aspects. 
ISSN : 2320-7353 
Journal Name : International Research Journal of Medical Sciences 
Volume : 3 
Issue Number : 1 
Publishing Year : 1436 AH
2015 AD 
Article Type : Article 
Added Date : Monday, April 1, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ماجد حمدي واكدWakid, Majed HamdiInvestigatorDoctoratemwakid@kau.edu.sa
عادل محسن أبوزنادةAbuzenadah, Adel MResearcherDoctorateaabuzenadah@kau.edu.sa
اشرف دلولDallol, Ashraf ResearcherDoctorateadallol@kau.edu.sa
جودة أحمد المغربيAl-Maghrabi, Jaudah AResearcherDoctoratejalmgrabi@kau.edu.sa

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