Document Details

Document Type : Article In Journal 
Document Title :
Evaluation of the Effects of Airborne Particulate Matter on Bone Marrow-Mesenchymal Stem Cells (BM-MSCs): Cellular, Molecular and Systems Biological Approaches
Evaluation of the Effects of Airborne Particulate Matter on Bone Marrow-Mesenchymal Stem Cells (BM-MSCs): Cellular, Molecular and Systems Biological Approaches
 
Document Language : English 
Abstract : Particulate matter (PM) contains heavy metals that affect various cellular functions and gene expression associated with a range of acute and chronic diseases in humans. However, the specific effects they exert on the stem cells remain unclear. Here, we report the effects of PM collected from the city of Jeddah on proliferation, cell death, related gene expression and systems of biological analysis in bone marrow mesenchymal stem cells (BM-MSCs), with the aim of understanding the underlying mechanisms. PM2.5 and PM10 were tested in vitro at various concentrations (15 to 300 µg/mL) and durations (24 to 72 h). PMs induced cellular stress including membrane damage, shrinkage and death. Lower concentrations of PM2.5 increased proliferation of BM-MSCs, while higher concentrations served to decrease it. PM10 decreased BM-MSCs proliferation in a concentration-dependent manner. The X-ray fluorescence spectrometric analysis showed that PM contains high levels of heavy metals. Ingenuity Pathway Analysis (IPA) and hierarchical clustering analyses demonstrated that heavy metals were associated with signaling pathways involving cell stress/death, cancer and chronic diseases. qRT-PCR results showed differential expression of the apoptosis genes (BCL2, BAX); inflammation associated genes (TNF-α and IL-6) and the cell cycle regulation gene (p53). We conclude that PM causes inflammation and cell death, and thereby predisposes to chronic debilitating diseases. 
ISSN : 1319-0989 
Journal Name : Arts and Humanities Journal 
Volume : 14 
Issue Number : 4 
Publishing Year : 1438 AH
2017 AD 
Article Type : Article 
Added Date : Sunday, July 9, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Muhammad Abu-ElmagdAbu-Elmagd, Muhammad InvestigatorDoctoratemabuelmagd@kau.edu.sa
Mansour A. AlghamdiAlghamdi, Mansour A.ResearcherDoctoratemans99@gmail.com
Magdy ShamyShamy, Magdy ResearcherDoctoratemshamy@kau.edu.sa
Mamdouh I. KhoderKhoder, Mamdouh I.ResearcherDoctoratekhoder_55@yahoo.com
Max CostaCosta, Max ResearcherDoctoratemax.costa@nyumc.org
Mourad AssidiAssidi, Mourad ResearcherDoctoratemourad.assidi@gmail.com
Roaa KadamKadam, Roaa ResearcherMasterrkadem@kau.edu.sa
Haneen AlsehliAlsehli, Haneen ResearcherMasterhalsehli@kau.edu.sa
Mamdooh GariGari, Mamdooh ResearcherDoctoratemgari@kau.edu.sa
Peter Natesan PushparajPushparaj, Peter NatesanResearcherDoctoratepeter.n.pushparaj@gmail.com
Gauthaman KalamegamKalamegam, Gauthaman ResearcherDoctoratekgauthaman@kau.edu.sa
Mohammed H. Al-QahtaniAl-Qahtani, Mohammed H.ResearcherDoctoratemhalqahtani@kau.edu.sa

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