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Document Type |
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Thesis |
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Document Title |
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The Antitumor Activity of Flaxseed Oil Nanoemulsions Incorporated with Sorafenib النشاط المضاد للورم للمستحلبات النانومترية لزيت بذور الكتان المدمجة مع سورافينيب |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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Sorafenib (SOR), a chemotherapeutic agent, is a multikinase inhibitor that has toxic effects which could limit its application in cancer therapy. The major objective of the present study was to load SOR in a nanoemulsion (NE) based on flaxseed oil (SOR- NE) and to evaluate the efficacy and cytotoxicity of the resulted formula in EAC-bearing female Swiss Albino mice. One hundred mice were divided into five groups (n =20). Groups I and II served as untreated mice and untreated EAC-bearing mice (2.5×106 EAC cells/mouse), respectively, while groups III-V represented the EAC-mice administered orally with blank NE, SOR dissolved in Cremophor (SOR-Chremo) and SOR- NE, respectively. The antitumor activity of the formulas was evaluated by detecting the efficacy of the administered drug through measuring the tumor volume and lactate dehydrogenase activity (LDH) in the ascetic fluids. The hepatotoxicity, cardiotoxicity, hematoxicity and nephrotoxicity were identified by determining the serum enzymes, complete blood count and studying the histological changes of the liver, heart and kidney tissues. The physical characteristics of SOR- NE, determined by the zetasizer and scanning electron microscope, exhibited that the z-average diameter of the nanodroplets was 77.46 ± 8.28 nm with negative zeta potential and a spherical morphology. It has been found that the LDH activity in the ascetic fluid of SOR-NE group has considerably enhanced with a significant decrease in tumor volume. SOR-NE has reduced the hepatotoxicity of SOR in mice with increased activity of the catalase enzyme. Furthermore, the reduction of hematoxicity and cardiotoxicity of SOR has been observed in SOR-NE group through lesser counts of white blood cells and decreased levels of cardiac enzymes. SOR-NE group has relatively normal liver, heart and kidney tissues when compared to the SOR-Chremo group. In conclusion, encapsulating SOR in NE has decreased its toxicity in the liver, heart and blood. |
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Supervisor |
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Dr. Maysoun Hosny AlKhatib |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1438 AH
2017 AD |
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Co-Supervisor |
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Dr. Maryam Abdu Al Ghamdi |
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Added Date |
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Thursday, June 8, 2017 |
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Researchers
| دنيا أحمد نوري | Nori, Dunya Ahmed | Researcher | Master | |
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