Document Details
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Document Type |
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Thesis |
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Document Title |
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BIOACTIVE METABOLITES FROM SAUDI MARINE INVERTEBRATES الأيضات النشطة بيولوجيا من اللافقاريات البحرية السعودية |
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Subject |
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Faculty of Sciences > Chemistry department |
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Document Language |
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Arabic |
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Abstract |
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The objective of the present work was the isolation of secondary metabolites produced by selected marine organisms (Soft coral) and/or algae, aiming at assessment of their biological activity. As, anti proliferative effect was evaluated. The effect of the potent compounds on the cell cycle was evaluated aiming at exploring the possible mechanism of action.
Two soft coral (Sarcophyton glaucum and Sarcophyton trocheliophorum) and red algae (Laurencia obtuse) were investigated. These investigations led to isolation and structure elucidation of fifteen pure compounds, representing different structural sub-classes within the terpenoidal derivatives (Sesquiterpenes and cembranoidal diterpenes).
Nine metabolites were isolated from Sarcophyton glaucum. Two of these are new cembranoidal derivatives; sarcoglauphine A (1) and sarcoglauphine A B (2) along with four known compounds; cembrene C (3), sarcophytol (4), ent. sarcophinediol (5), sarcotrocheliol acetate (6) and sarcotrocheliol (7); 10(14)-aromadendrene (8), and deoxosarcophine (9).
Three metabolites were isolated from Sarcophyton trocheliophorum. One of these was identified as a new tetracyclic biscrembrene, Trocheliane (10), along with two new cembranoidal derivative; sarcotrocheliol A (11) and sarcotrocheliol B (12) were isolated for the first time from Sarcophyton trocheliophorum.
A new eudesmane sesquiterpenoid, eudesma-4(15),7-diene-5,11-diol (13) along with the known trinor-sesquiterene, teuhetenone (14), and a seco-eudesmane sesquiterpene, chabrolidione B (15), were isolated from the red algae, Laurencia obtusa.
The structure of all the metabolites were elucidated using different spectroscopic techniques (NMR, MS, IR and UV) and/or comparing with the previously reported data. Compounds (3-9), showed significant cytotoxic activity with IC50 values in range 9.9±0.03 to 20.0±0.068 µM, while 13 and 15 showed significant cytotoxicity against MCF-7 with IC50 values 2.9 µM. Finally, compound 14 showed moderate antiproliferative effect against MCF-7 with IC50 value 22 µM. The sensitivity of a number of pathogenic bacteria used as test organisms to compounds (10-12) was carried out. Compound 10 showed good antimicrobial activity with diameter of inhibition zones ranged from 11-18 mm. |
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Supervisor |
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PROF.DR. Khalid Omar Al-Footy |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1437 AH
2016 AD |
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Added Date |
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Monday, June 13, 2016 |
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Researchers
| محمد هالد هاتير سلطان | P H, MOHAMED HALID | Researcher | Doctorate | |
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