Document Details

Document Type : Article In Journal 
Document Title :
Isolation and Genotyping of Cryptosporidium among HIV
عزل ودراسة جينية لطفيلي الكريبتوسبوريديوم من المصابين وغير المصابين بفيروس نقص المناعة البشرية المكتسبة في جدة، المملكة العربية السعودية
 
Subject : Diagnostic Medical Parasitology 
Document Language : English 
Abstract : Cryptosporidium is an opportunistic apicomplexan parasite that can cause severe diarrhea in children and immunocompromised persons. In this study, 210 stool samples were collected from adult and children patients, of which 47% are HIV-infected patients in Saudi Arabia. Cryptosporidium identification was performed by both modified Kinyoun acid fast staining and molecular analysis using nested polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Characterization in our analysis was setup on the amplification of a DNA fragment from the Cryptosporidium oocyst wall protein (COWP) gene followed by digestion with RsaI restriction enzyme. Nested PCR based on SSU-rRNA product after digestion with SspI and VspI restriction enzymes was also performed. A total of three samples (1.43%) were positive in both methods. Two samples were identified as Cryptosporidium parvum and the third one as Cryptosporidium hominis. This is the first study in Saudi Arabia to characterize Cryptosporidium in HIV-infected persons. Our finding does not exclude the zoonotic transmission of the disease and further studies are essential for better understanding for the anticipated risk factors and sources of infection in Saudi Arabia. 
ISSN : 1817-3055 
Journal Name : World Journal of Medical Sciences 
Volume : 11 
Issue Number : 1 
Publishing Year : 1435 AH
2014 AD 
Article Type : Article 
Added Date : Saturday, January 24, 2015 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ماجد حمدي واكدWakid, Majed HInvestigatorDoctoratemwakid@kau.edu.sa
عصام إبراهيم أزهرAzhar, Esam IResearcherDoctorateEazhar@kau.edu.sa
أحمد عبدالله الغامديAl-Ghamdi, Ahmad AResearcherDoctorateaalghamdi@kau.edu.sa
حنان عبدالرحمن بخشBakhsh, Hanan AResearcherDoctoratehanaa.bakhsh@yahoo.com
حنان محمد أيوبAyoub, Hanan MResearcherDoctoratehayoub@yahoo.com

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