Document Details

Document Type : Thesis 
Document Title :
Prevalence Study of Hepatitis B virus (HBV) Infection by Molecular & Serological techniques in Jeddah province
دراسة إنتشار الإصابة بفيروس إلتهاب الكبد الوبائي(بي) بواسطة التقنيات الجزئية والمصلية في محافظة جدة
 
Subject : biological sciences department 
Document Language : Arabic 
Abstract : Chronic liver disease is an important health challenge in the world, where hepatitis B virus (HBV) infections are the main causes of liver insufficiency. HBV infection is a serious global health problem, with two billion people infected worldwide, and 350 million suffering from chronic HBV infection. The objective of this study was prevalence of Hepatitis B virus on Jeddah populations, Saudi Arabia. (HBV) was screened in serum and plasma by using Elisa test and Molecular techniques (PCR), samples were collected and stratified according to nationality into Saudi and non Saudi and according to gender into male and female and also according to age. Prevalence was calculated separately for each group and for studied population as a whole. Results showed that HBsAg were found in 6.11 % seropositive of sample in study populations, the prevalence of HBs Ag was higher in males than females it is 8 % among males and 5.61 % among females with significant difference (p<0.05). Also HBs Ag were found in 6.53 % of Saudi population and 1.79 % of non Saudi patient with no statistically significant difference (p>0.05). According to age results all positive samples were found in adult age while no positive sample at age under 15 years with no significant difference (p>0.05) . Hepatitis B DNA was confirmed by polymerase chain reaction (PCR) was tested by three different primers (conserved region, HBx gene and HBs gene) and PCR positive products were made sequencing. The results made align with HBV gene to find out the relationship between the genotypes in the selected patients samples with the genotyping of HBV. This study also indicated to the predominant genotype of HBV in Jeddah is belonged to genotype B, C, D and subgenetype adr. Finally in this study we tried to draw attention through the study to create future strategies to deal with this virus and limit its spread in Saudi society. 
Supervisor : Prof. Dr. Mohamed Morsi Mohamed Ahmed 
Thesis Type : Doctorate Thesis 
Publishing Year : 1434 AH
2013 AD 
Added Date : Wednesday, May 29, 2013 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
محمد بكر برناويBarnawi, Mohammed BakerResearcherDoctorate 

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