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Document Type |
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Thesis |
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Document Title |
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Comparison Between Different Phenotypic and Genotypic Methods for Detection Of Metallo β-Lactamase in Pseudomonas aeruginosa that has Multi-drug Resistance to Antibiotics المقارنة بين طرق التنميط المظهرية و الوراثية المختلفة للكشف عن إنزيمات Metallo β-Lactamase في بكتيريا Pseudomonas aeruginosa ذات المقاومة المتعددة للمضادات الحيوية |
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Subject |
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biological sciences department |
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Document Language |
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Arabic |
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Abstract |
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Since their discovery during the 20th century, antimicrobial agents have substantially reduced the threat posed by infectious diseases, it has led to a dramatic drop in deaths from diseases that were previously widespread, untreatable, and frequently fatal. But now the Antibiotic resistance is a worldwide public health problem that continues to grow. One part of the problem is that bacteria and other microbes that cause infections are remarkably resilient and have developed several ways to resist antibiotics. Nowadays, about 70% of the bacteria that cause Health Care Associated Infections are resistant to at least one of the drugs most commonly used for treatment. Some organisms are resistant to all approved antibiotics and can only be treated with experimental and potentially toxic drugs. Infection-causing bacteria that were formerly susceptible to an antibiotic can develop resistance through changes in their genetic material, or deoxyribonucleic acid (DNA). Bacteria are particularly efficient at enhancing the effects of resistance, not only because of their ability to multiply very rapidly but also because they can transfer their resistance genes, which are passed on when the bacteria replicate. The Pseudomonas aeruginosa is the example of Multiple Drug resistance Bacteria and it is a significant opportunistic pathogen and a major cause of HAIs that have multi drug resistant for β-Lactam antibiotics. Resistant to antipseudomonal-β-Lactams may arise via hyperproduction of an IMP Metallo-β-Lactamase enzyme. At present, 21 IMP MBLs variants have been detected and it is increase producing. MBLs is a major cause of antibiotic resistance by hydrolyzing β-Lactam antibiotics. This study was designed to evaluate the best method to rapid and accurate detection of IMP-1 as a routinely procedure in Microbiology laboratory to implement adequate infection control measures to prevent its spread specially at hospitals by using two phenotypic methods ( EDTA, Etest) and PCR amplificition method as genotypic detection on 34 isolates of P. aeruginosa that were shown resistance to Imipenem and Meropenem from 174 isolates of P. aeruginosa that isolated from King Abdulaziz Hospital and Oncology Center, Jeddah during one year, and comparison between this methods to determine which the more rapid and accurate method to detection of MBLs. The results were shown that the intensive care unit was the most of the source of infection (25.3%), and the great majority according to the site of infection was obtained from wounds swab (32.8% ), the results also were shown that P. aeruginosa was observed high rates of multi drug resistance where 23.5% were resistant to Imipenem and 22% were resistant to Meropenem, and 6% of isolates were contained IMP-1 gene. The Etest strip method was more accurate method to detection of MBL where it depends on determine the minimal inhibitory concentration. In conclusion, this study was shown that the prevalence of HAIS by P. aeruginosa that had multi drug resistant was high among patients who were admitted to the ICU. Accordingly, the spread of infection should be prevent by the implement adequate infection control measures. |
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Supervisor |
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Dr. Ahmed Mahmoud Alhejin |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1433 AH
2012 AD |
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Co-Supervisor |
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Dr. Hani Zakaria Asfour |
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Added Date |
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Monday, July 9, 2012 |
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Researchers
| انعام خالد ادريس | Idrees, Enaam Khalid | Researcher | Master | |
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