Document Details

Document Type : Article In Journal 
Document Title :
Toxicity , Histopathological and Ultrastructural effects of Artemisia hrba alb extract in the Midgut of Culex quinquefasciatus larvae (Diptera : Culicidae)
Toxicity , Histopathological and Ultrastructural effects of Artemisia hrba alb extract in the Midgut of Culex quinquefasciatus larvae (Diptera : Culicidae)
 
Subject : Toxicity , Histopathological and Ultrastructural effects of Artemisia hrba alb extract in the Midgut of Culex quinquefasciatus larvae (Diptera : Culicidae) 
Document Language : English 
Abstract : Crude ethanolic extracted from Artemisia herba alba,,was tested against 3rd larval instar of Culex quinquefasciatus .The larvae were exposed to serial concentrations (2.75, 2.50, 2.25, 2.00, 1.50 & 1.00 mg/l). The plant extract showed marked effects on larval mortality.sequental observations of the light and ultrastructural effects of LC50 value were made on gastric ceacae and midgut epithelial cells .Exposure times (6 , 12 , 24 and 48 hours) were tested.The toxic effect of the extracts have wide rang of affecting degrees in the elementary canal . The main alterations observed in gastric caecae and midgut were partial or total cell destruction,high cytoplasmatic vacuolization, disruption of rough endoplasmic reticulum ,cell hypertrophy and the epithelium did not maintain its monolayer appearance. At increased time the midgut epithelium cells from treated larvae appeared more elongate and swollen than cells observed from control larvae .the microvilli was degenerated. After 48 hours the changes more deep, with the epical region of the cell bulging into the gut lumen. The cells contained large cytoplasmic spaces, cellular damage had increased ,with cells ruptured at the apical surface and leakage of cytoplasmic materials into the gut lumen.Observation of tissues sections from larvae treated for different length of time revealed a wide variation in the degree of damage. The present study provides evidence regarding the mode of action of the A. herba alba extract used as bioinsecticidal against mosquito larvae. 
ISSN : 1012-1319 
Journal Name : Science Journal 
Volume : 54 
Issue Number : 1 
Publishing Year : 1430 AH
2009 AD 
Article Type : Article 
Added Date : Thursday, May 31, 2012 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
رقية محمد المحماديAlmehmadi, Roqaiah MohammadInvestigatorDoctorated_almehmadi@yahoo.com
أريج الخلفalkhalf, Areej ResearcherDoctorateAj_khalaf@yahoo.com

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