Document Details

Document Type : Article In Journal 
Document Title :
Relative quantitation of HIV-1 proviral DNA amplified using the polymerase chain reaction
Relative quantitation of HIV-1 proviral DNA amplified using the polymerase chain reaction
 
Document Language : English 
Abstract : Abstract: A 141-base pair fragment of human immunodeficiency virus-1 (HIV-1) DNA was amplified using the polymerase chain reaction (PCR). The products were slot-blotted onto a nitrocellulose membrane, revealed with a digoxigenin-labelled probe and quantitated by scanning densitometry. This method for the relative quantitation of HIV-1 DNA achieved reliable results and avoided the use of radioisotopes and the electrophoretic transfer of DNA. Testing of serial dilutions of HIV-1 extracted from infected cells revealed smooth titration curves. A reproducible increase in peripheral blood HIV-1 DNA was documented in a haemophilia patient during disease progression. 
ISSN : 0306-4522 
Journal Name : Serodiagnosis and Immunotherapy in Infectious Disease 
Volume : 6 
Issue Number : 4 
Publishing Year : 1414 AH
1994 AD 
Article Type : Article 
Added Date : Sunday, May 30, 2010 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
آصف جان مان فطانيJiman Fatan, Asif ResearcherDoctorate 

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