Document Details
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Document Type |
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Article In Journal |
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Document Title |
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EFFECT OF PH AND METAL IONS ON THE ANTIADHERENT ACTIVITY OF LEVOFLOXACIN AGAINST PREADHERENT PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS EFFECT OF PH AND METAL IONS ON THE ANTIADHERENT ACTIVITY OF LEVOFLOXACIN AGAINST PREADHERENT PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS |
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Document Language |
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English |
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Abstract |
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ABSTRA CT
The effects of metal ions (Ca++ and Mg++) and pH (5.5-8.5) on the antiadherent activity of levofloxacin against preformed biofilms of Pseudomonas aeruginosa and Staphylococcus aureus clinical isolates ( 12 isolates each), in wells of microtiter plates and on segments of vascular catheters using an in vitro model of catheter colonization, were studied. The presence of 5 mMg increased the percentage of optical density of the adherent biofilms of P. aeruginosa and S. aureus treated with levofloxacin (12.5-200 ug/ml,) by 16-29% and 22-40%, respectively, comparing to that of the control. In the presence of 5 mM Ca++, no significant (P < 0.05,) change was observed. A slight decrease in the percentage of optical density of the adherent biofilms of P. aeruginosa and S. aureus isolates treated with levofloxacin at pH-5.5 by 9-12% and 11-15%, respectively, was observed as compared to that obtained at pH 7. While at pH 8.5, the percentage of optical densities of the tested isolates was slightly increased by 8-12% and 8-10%, respectively. In the in vitro model of vascular colonization, the presence of 100 µg/ml levofloxacin eradicated all the adherent cells. A few colonies of the tested isolates was recovered in the presence of 5 mµMg or at pH 8.5. While, no significance difference was observed in the presence of 5 mM Ca or at pH 5.5. Treatment of the catheter segments with 50 µg/ml levofloxacin reduced the range of the number of tile adherent cells to 15-22 CFU/ml. The presence of 5mMg’ (plus levofloxacin,) increased the range of the mean number of the adherent cell to 2 7-38 CFU/ catheter segment as compared to that of the controls (preadhered films treated with levofloxacin,), while in the presence of 5 mM Ca++ no significance differences (P < 0.05) in the number of the adherent cells was observed. Concerning the effect of pH, it was observed that the range of the number of the CPU/catheter segment at pH 5.5, 7.0, and 8.5 was 10-16, 15-22, and 28-36, respectively. Accordingly, the presence of Mg++ and pH 8.5 slightly decreased the antiadherent activity of levofloxacin. In contrast, the antiadherent activity of levofloxacin was increased at pH 5.5. In case of Ca++, no significant effect on the antiadherent activity of levofloxacin was observed. |
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ISSN |
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17852933 |
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Journal Name |
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N. Egypt. J. Microbiol. |
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Volume |
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15 |
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Issue Number |
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2 |
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Publishing Year |
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1426 AH
2006 AD |
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Article Type |
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Article |
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Added Date |
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Sunday, May 30, 2010 |
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Researchers
| سليمان الشيخ | EL-sheikh, Soliman | Researcher | Doctorate | |
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