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Document Type |
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Thesis |
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Document Title |
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Studies on the antagonistic effect of some bacteria isolated from human milk against Staphylococcus aureus دراسات على التأثير التضادي لبعض أنواع البكتيريا المعزولة من لبن نساء مرضعات ضد بكتيريا Staphylococcus aureus |
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Subject |
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College of Science for Girls |
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Document Language |
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Arabic |
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Abstract |
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This research aims at finding bacteria, Which produce materials, that inhibit growth of disease causing and food poisoning bacteria, such as bacteriocins, from natural resources as nursing women, through isolating bacteria producing materials that inhibit growth of Staphylococcus aureus bacteria and comparing their counter activity with counter activity of some of bacteria types isolated from goats’ milk, 4.414 types were obtained from 43 samples from women milk and 162 types from goats’ milk from Baha Region during different periods of milk production. Most of the isolated bacterial types showed ability to inhibit growth of the tested bacteria, S. aureus by the method of patching solid medium. Twelve isolated bacteria were chosen from the lactic acid bacteria, seven from women milk and five from goats’ milk from subsequent studies. The filtrate of the selected isolated bacteria was found to inhibit growth of S. aureus after removing the effect of hydrogen peroxide and acidity from the filtrate. While the highest antibacterial activity against S. aureus in the liquid medium after 168 hours of incubation at 35◦C in an MRS medium. When the dextrose in the MRS medim was substituted, with one of the following molasses types: a mixture of dates and sukkari dates molasses, Qassim dates and crush dates with a concentration of 2% as a source of sugars, the production of most of the isolated bacteria of the materials that inhibit growth of the tested bacteria; while use of a medium of 2% of molasses or crushed dates as a single medium for on the antibacterial activity against of S. aureus test bacteria (bacteriocins) by the selected isolates bacteria types, all isolated bacteria types were able to inhibit growth of S. aureus with quantities comparable with their ability to produce bacteriocins in an MRS medium. The best concentration of molasses types and crushed dates form getting the highest antibacterial activity that inhibit growth of the selected bacteria was 2%. However, antibacterial activity growth of the test bacteria was reduced aftar addition of twin -80 only, with a concentrate of yeast and twin-80 added together and when adding peptone and twain – 80. Moreover, the antibacterial activity of isolated bacteria inhibity growth of S. aureus when adding all those materials together to the dates media is equivalent to its production of the inhibiting materials when using the MRS medium alone. It was also found that the antibacterial activity inhibit growth of S. aureus in filtrate is reduced when subjected to the boiling point temperature for 3 minutes compared to the control. |
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Supervisor |
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dr. |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1431 AH
2010 AD |
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Added Date |
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Tuesday, May 18, 2010 |
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Researchers
| عفراء محمد بغدادي | Bagdadi, Afraa Mohammad | Researcher | Master | |
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