Document Details

Document Type : Project 
Document Title :
Comparison of Microscopy and PCR for Detection and Identification of Cryptosporidium Species
مقارنة بين الفحص المجهري واختبار تفاعلات البلمرة التسلسلي لتشخيص طفيلي الكريبتوسبوريديوم
 
Subject : Diagnostic Medical Parasitology 
Document Language : English 
Abstract : Cryptosporidium is an opportunistic intestinal protozoa that infects humans causing chronic watery diarrhea and could be life threatening in immunocompromised patients and children. Water out-breaks of cryptosporidiosis have been widely reported. Microscopic examination of acid fast stained smears is considered as the most common routinely technique for diagnosis of Cryptosporidium oocysts. In a previous study with the above stain, we found that most of the samples were positive for oocysts. This result need confirmation as if oocysts of other acid fast organisms could show same size and reaction with the stain. Polymerase chain reaction (PCR) is highly sensitive and specific for detection and identification of organisms. In this study we will diagnose Cryptosporidium in fecal samples from patients in Jeddah by different technique of PCR and compare it with the acid fast technique. 
Publishing Year : 1429 AH
2008 AD 
Sponsor Name : King Abdulaziz University 
Sponsorship Year : 1429 AH
2008 AD 
Added Date : Monday, February 22, 2010 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ماجد حمدي واكدWakid, Dr. Majed HInvestigatorDoctoratemwakid@kau.edu.sa

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