Document Details
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Document Type |
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Thesis |
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Document Title |
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Biochemical and Histological Studies on the effect of Zearalenone Mycotoxin and Thymoquinone on Male Mice Kidney دراسات كيموحيوية ونسيجية على تأثير السم الفطري زيرالينون والثيموكوينون على كلى ذكور الفئران |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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The contamination of food and feedstuff with mycotoxins represents a worldwide problem for both humans and animals. Zearalenonen is a mycotoxin produced by Fusarium species has estrogenic and anabolic activity. It is one of the most widely distributed fusarial mycotoxins, which is encountered at high incidence in many important corps likes corn, wheat, barley, oats, sorghum and sesame which intended for human and animal consumptions. In this study, nephrotoxicity of ZEN was evaluated in male adult Balb/C mice, given single and repeated doses of ZEN mycotoxin (2.7 mg/kg B.W) via oral route. The protective effect of Thymoquinone extracted from Nigella Sativa was also assessed. Mice were divided into four groups (N= 10). G1: receiving the toxin once and dissected 48h later, G2: given the toxin twice a week for one week, G3: given the toxin twice a week for two weeks, G4: pretreated orally by Thymoquinone (10 mg/Kg B.W) for 7 days prior to administration of ZEN twice a week for two weeks. Each treated group has its corresponding control which received 1% DMSO saline.
The Serum level of blood urea nitrogen (BUN), creatinine (CRE), alpha-fetoprotein (AFP), pyruvate kinase isoenzyme tumor M2 (Tu M2_PK) and total antioxidant status (TAS) was assessed. The level of BUN was increased significantly in serum in one and two weeks compared to control but the level of CRE was deceased significantly in one and two weeks compared to control. Also, the activity of TAS was increased in 48 Hours, one and two weeks compared to control. Although the concentration of AFP was increased significantly in two weeks compared to control, the concentration of Tu M2_PK was increased significantly in all group compared to control. The recovery occurred in the TQ group based on changes in the level of (BUN, CRE, AFP, Tu M2_PK, TAS) when comparing with G3 which treated by ZEN only. Histological changes of mice kidney coincided and confirmed with biochemical changes. In conclusion, this study revealed that ZEN induced changes in kidney function and structure. The Thymoquinone from Nigella sativa might be used as new prophylactic approach to enhance kidney cells detoxification of ZEN mycotoxin. |
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Supervisor |
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Prof. Najwa M. EL-Sawi |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1431 AH
2010 AD |
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Co-Supervisor |
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Dr. Madeha N. Al-Seeni . Dr. Soad S. Ali |
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Added Date |
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Monday, August 23, 2010 |
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Researchers
| سلمى محمد الجحدلي | AL-Jahdali, Salma Mohammed | Researcher | Master | |
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